Composite

Part:BBa_K1460004:Design

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-26)


Anderson Promoter + MerT + MerP + ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 92


Design Notes

The Anderson promoter provides strong constitutive expression of merT/merP in E. coli

Source

Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa.[1] This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.

References

[1] Diver, W., Grinsted, J., Fritzinger, D., Brown, N., Altenbuchner, J., Rogowsky, P., & Schmitt, R. (1983). DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and Tn1721. Molecular & General Genetics, 189-193.